UCLA Metabolomics Shared Resource

Our center has an emphasis on metabolomics, proteomics related to metabolism, and other proteomics projects. Our emphasis is on collaborative projects that push mass spectrometry-based methods forward, and on sharing these advances with faculty at UCLA and at other top-ranking universities throughout the country.

Overview of Facility History

The UCLA Metabolomics and Proteomics Shared Resource was established in the spring of 2013. The mission of the center is to develop and apply cutting-edge approaches for quantitative metabolomics and proteomics.

About our services

Our facility is collaborating with users from over 40 different labs, including 34 at UCLA, 6 labs off campus (Caltech, Harvard, Stanford, UCSD, UCSF and USC) and one lab at the Max Planck Institute in Freiburg, Germany.

The nature of the projects is very diverse, ranging from metabolite level measurements in organelles (eg. liver mitochondria) to mouse blastospheres and from determining changes in cell culture medium (metabolic footprint) to metabolic differences in blood and tumors from human brain trauma patients and transgenic mice, respectively.
For an introduction into sample preparation, our analysis pipeline and output as well as for an overview of the types of samples we have prepared previously, please click here.

Instruments

Data is collected using two Thermo Scientific Q Exactive mass spectrometers coupled to a UltiMate 3000 UPLC chromatography systems, which allows us to look for metabolites and proteins in a targeted (SIM) and untargeted (full scan) approach. In addition, this instrument allows for fragmentation of selected ions, which allows us to sequence peptides and analyze the structure of small molecules.