To discuss your project with us, contact Johanna ten Hoeve-Scott <firstname.lastname@example.org>
Johanna ten Hoeve-Scott
4338 CNSI, UCLA
570 Westwood Plz
Los Angeles, CA, 90095
The UCLA Metabolomics Center is endorsed by the UCLA Clinical and Translational Science Institute (CTSI).
Apply for vouchers here: http://ctsi.ucla.edu/funding/pages/fund
Refer to our protocols for sample preparation:
- Cell metabolite extraction protocol.pdf
- Metabolite extraction from cell culture medium.pdf
- Metabolite extraction from blood plasma and serum.pdf
- Metabolite extraction from CSF.pdf
- Tissue metabolite extraction protocol.pdf
- Cecum Metabolite Extraction.pdf
Cells are usually plated onto 6well plates. The targeted cell number at the time of extraction is >= 200k cells / well. For metabolite extraction, we prefer a 1-phase method (MeOH only), but a 2-phase extraction (including CHCl3) works well, also.
For simplicity, please label your sample tubes with 1, 2, 3, etc and fill out the sample information sheet accordingly. Please save the sample information sheet with the name of the study including a date.
Please fill out and email the sample information sheet to Johanna when you submit your samples. Please remember to check if the funding information is up-to-date. For UCLA clients, please provide a valid recharge ID or FAU. Once you have filled out the sample info sheet, please save the .xlsx file with the name of the study including a date. Please be careful with leading and trailing spaces in the condition names as this will cause the same conditions to appear as distinct conditions in our scripts and lead to more work for us.